Prema Singh
In this study, we created and validated a straightforward, quick, and sensitive LC-MS/MS approach for tetrodotoxin detection in human plasma. We compared three solid phase extraction carriers of the HILIC type with various stationary phase functional groups. The Siphila HILIX SPE plate with multi-carboxyl groups was ultimately chosen because it had a clearly superior extraction recovery of TTX (about 80% of recovery from plasma samples) compared to the other two, and because no significant matrix effects were noticed. This was thought to be the result of mixed-mode synergistic effects of hydrophilic interaction and ion exchange. Acetonitrile with 1% trichloroacetic acid was used to quickly precipitate a 100 mL sample of plasma, and the filtrates were then put onto a Siphila i HILIX 96 well SPE plate. TTX was extracted with 200 mL of 50% acetonitrile after being washed with 95% acetonitrile. LC-MS MS and the whole run duration on a BEH amide column were both immediately injected with trichloroacetic acid. Of the elution solution. The procedure skips evaporation and ultrafiltration, which is straightforward and efficient. In positive mode, and were used to monitor the internal standard and TTX, respectively. The intra- and inter-assay accuracies of the method were in the ranges of 98.5%-99.8% and 98.8-99.5%, respectively, with the low limit of quantification. The method's good anti-interference property was demonstrated by the fact that biases of spiking analysis varied from -7.00% to 7.43% for healthy human plasma samples (RSDs 8.83%) and from -5.00% to 3.93% for hemolytic, high triglyceride, high cholesterol, and high bilirubin plasma samples. Human health is seriously threatened by tetrodotoxin. LC-MS MS and the whole run duration on a BEH amide column were both immediately injected with trichloroacetic acid of the elution solution.
KeywordsTetrodotoxin; Hydrophilic-interaction/ion-exchange; Mixedmode solid phase extraction; LC-MS/MS; Human plasma
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