Yuri Sarma
Our aim was to understand and analyse the expression intensity of selectively a singular gene (in our case, FANCC) which is a part of the DNA damage response pathways associated with cervical cancer. We performed
immunohistochemistry of six different slides obtained from CNCI hospital, out of which 3 were normal samples, while three were tumour samples. We analysed the expression intensity pattern for BRCA1, BRCA2, FANCC, FANCD2, DNMT1, DNMT2, MLH1 and MLH2 and primarily focused on FANCC in this report.
FANCC (Fanconi Anaemia complementation group)/FANC is a damage repair gene associated with the Homologous Recombination Repair pathway (HRR) that provides specific instructions for making a particular protein that is involved in one of the fanconi anaemia pathway. The FA pathway is activated when the process of DNA replication is blocked due to DNA damage, specifically interstrand crosslinks.
Immuno Histo Chemistry (IHC) is a laboratory method for detecting antigens in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues. IHC is widely used in many research and clinical laboratories because this technique makes it possible to visualize the distribution and localization of specific cellular components within cells.
It was observed that protein predominantly localizes around the nucleus in normal samples whereas it localises around the nucleus and cytoplasm in tumour samples. Additionally, the intensity of expression increases from basal-parabasal to spinous in normal samples and intensity of expression decreases as a whole in tumour samples. This further indicated that the stem cell property of basal parabasal of normal cervical epithelium is reflected in cervical lesion sample. This opens for further investigation where we can link the high expression activity of FANCC with progression cervical cancer. This will give us new therapeutic targets and eventually can be used for medical purposes in the future.
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